| 產品編號 | bs-1449R |
| 英文名稱 | Rabbit Anti-Prohibitin antibody |
| 中文名稱 | 阻抑素/腫瘤抑制因子抗體 |
| 別 名 | Mitochondrial Marker; PHB; PHB1; Prohibitin - Mitochondrial Marker; PHB_HUMAN. |
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Specific References (2) | bs-1449R has been referenced in 2 publications.
[IF=5.23] Wang, Jinzi, et al. "Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus." Scientific Reports 6 (2016): 34308. WB ; Other Species.
[IF=2.08] Xing, Wen Min, et al. "Proteomic identification of mitochondrial targets involved in andrographolide sodium bisulfite-induced nephrotoxicity in a rat model."Environmental Toxicology and Pharmacology (2015). WB ; Rat.
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| 研究領域 | 腫瘤 細胞生物 染色質和核信號 細胞凋亡 細胞類型標志物 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=2ug/test,ICC/IF=1:100,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Prohibitin: 171-272/272 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Function: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. Subunit: Interacts with PHB2. Interacts with STOML2. Subcellular Location: Mitochondrion inner membrane. Tissue Specificity: Widely expressed in different tissues. Similarity: Belongs to the prohibitin family. SWISS: P35232 Gene ID: 5245 Database links: Entrez Gene: 5245 Human Entrez Gene: 18673 Mouse Omim: 176705 Human SwissProt: P35232 Human SwissProt: P67778 Mouse Unigene: 514303 Human Unigene: 263862 Mouse Unigene: 29754 Rat Prohibitin(phb)基因是一種有效的腫瘤抑制基因，廣泛分布于不同種屬的各種生物細胞中，它所編碼的產物Prohibitin(PHB)蛋白結構保守，既存在于線粒體內膜上，發(fā)揮分子伴侶作用，也存在于細胞核內，具有負性轉錄調控作用，因而對細胞代謝、生長、分化、衰老以及凋亡等諸多方面發(fā)揮 著重要的調控作用，可能與某些腫瘤和退行性疾病的發(fā)生有關。 |
| 產品圖片 |
Sample:
Heart(Mouse) Lysate at 40 ug
Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Sample:
A431(Human) Cell Lysate at 40 ug
Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Prohibitin) polyclonal Antibody, Unconjugated (bs-1449R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
