| 產(chǎn)品編號 | bs-1273R |
| 英文名稱 | Rabbit Anti-E-Selectin antibody |
| 中文名稱 | E選擇素抗體 |
| 別 名 | CD 62E; CD26E; CD62E antigen; CD62 antigen-like family member E; E selectin; ELAM 1; ELAM; ELAM-1; ELAM1; Endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; LECAM 2; LECAM2; Leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; LYAM2_HUMAN; SELE; Selectin E; SelectinE. |
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Specific References (17) | bs-1273R has been referenced in 17 publications.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞粘附分子 細(xì)胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65kDa |
| 檢測分子量 | 115 |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse E-Selectin: 501-612/612 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008] Function: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Subunit: Interacts with PSGL1/SELPLG through the sialyl Lewis X epitope. PSGL1 sulfation appears not to be required for this interaction. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Belongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 6 Sushi (CCP/SCR) domains. SWISS: Q00690 Gene ID: 20339 Database links: Entrez Gene: 6401 Human Entrez Gene: 20339 Mouse Omim: 131210 Human SwissProt: P16581 Human SwissProt: Q00690 Mouse Unigene: 82848 Human Unigene: 5245 Mouse Unigene: 10359 Rat E選擇素(CD62E)是活化的內(nèi)皮細(xì)胞表面表達(dá)的黏附分子,是白細(xì)胞與內(nèi)皮細(xì)胞形成接觸時(shí)的啟動因子。E選擇素是表達(dá)在血管內(nèi)皮細(xì)胞表面的黏附分子。 |
| 產(chǎn)品圖片 |
100 ng rhE-Selectin-His protein (bs-43646P) per lane probed with E-Selectin polyclonal antibody respectively, unconjugated (bs-1273R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with E-Selectin Polyclonal Antibody, Unconjugated (bs-1273R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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