| 產(chǎn)品編號 | bs-0610R |
| 英文名稱 | Rabbit Anti-Adiponectin Receptor 1 antibody |
| 中文名稱 | 脂聯(lián)素受體1抗體 |
| 別 名 | ACDCR1; ADIPO R1; Adiponectin receptor protein 1; ADIPOR 1; ADIPOR1; CGI-45; CGI-45 protein;FLJ25385; PAQR1; Progestin and adipoQ receptor family member I; TESBP1A; ADR1_HUMAN. |
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Specific References (14) | bs-0610R has been referenced in 14 publications.
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| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 生長因子和激素 糖尿病 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Adiponectin Receptor 1: 241-270/375 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Acrp30 is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR- . Cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) have shown that AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. Function: Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin. Subunit: May form homo and heteromultimers. Subcellular Location: Membrane; Multi-pass membrane protein. Note=Localized to the cell membrane and intracellular organelles. Tissue Specificity: Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine. Similarity: Belongs to the ADIPOR family. SWISS: Q96A54 Gene ID: 51094 Database links: Entrez Gene: 51094 Human Entrez Gene: 72674 Mouse Omim: 607945 Human SwissProt: Q96A54 Human SwissProt: Q91VH1 Mouse Unigene: 5298 Human Unigene: 259976 Mouse 脂聯(lián)素與受體結(jié)合后具有增強胰島素敏感性，抗高血糖，抗動脈粥樣硬化等生物學(xué)效應(yīng)，任何增加或減少脂聯(lián)素及其受體表達(dá)的方法都影響這些疾病的發(fā)生和發(fā)展. 脂聯(lián)素受體-1(AdipoR1和AdipoR2)同屬于PAQR家族，具有七次跨膜蛋白的特有結(jié)構(gòu)， mol wt:41kDa |
| 產(chǎn)品圖片 |
Sample: Muscle(Mouse) Lysate at 40 ug
Primary: Anti- Adiponectin Receptor 1 (bs-0610R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Breast tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Rat Muscle tissue lysates
Lane 5: Rat Heart tissue lysates
Primary: Anti-Adiponectin Receptor 1 (bs-0610R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated(bs-0610R) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated(bs-0610R) 1:300, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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